Contenido (Unidad /
Competencia / Actividad / Material de Aprendizaje) |
1. Introducción
1.1. Terminología empleada
1.1.1. Cultivo de tejido
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, p. 459
Pierik, R. L .M., (1990). Cultivo in vitro de plantas superiores. Ed. Multi-prensa. p. 6-7
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlandsp. 341-346
1.1.2. Terminología empleada en cultivo de tejidos
Buchanan B. B, Gruissem W y Jones RL (2000). Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, Maryland, USA. cap I, 3-15
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, 459
Pierik, R. L .M., (1990). Cultivo in vitro de plantas superiores. Ed. Multi-prensa. p. 6-20
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlandsp. 459
1.1. Historia del cultivo in vitro
1.1.1. Historia del cultivo de tejidos
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, 1-14
Pierik, R. L .M., (1990).Cultivo in vitro de plantas superior es. Ed. Multi-prensa, p. 3-5.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlandsp. 344
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia., p. 3-7
1.2. Tendencias futuras
1.2.1. Aplicaciones de las tecnicas de cultivo de tejidos
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 346-348.
Serrano, M., y Piñol, M. T. 1991. Biotecnología vegetal. Ed. Síntesis, 359
Green, C:E. Somers, D:A:, Hackett, W:P:, Biesboer, D-D. eds. (1978). Plant Tissue and Cell Culture, Alan, R., Liss, INC. International Congress on plant tissue and Cell culture (6th: 1986: University of Minnesota) Plant Cell and Cell Culture (Plant Biology: v 3.Printed In the united states of América,Library of Concress Cataloging in publication, Data.p. 435-495.
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2. Organización y diseño de un laboratorio
2.1. Areas de un laboratorio
2.1.1. Secciones de un laboratorio de cultivo de tejidos
Hartmann, H.T., Kester, D.E., Davies, F.T.(1990). Plant Propagation, Principles and Practices, Fifth Edition. Regents prentice Hall, Englewood Cliffs, New jersey 07632. Printed in Unites States of America, 647.
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, p. 459,461,497.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlandsp. 31-42
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 4-11
2.2. Líneas de alimentación (gas, agua. aire comprimido, líneas de aire y vació etc.)
2.2.1. lineas de alimentación de servicios
12. Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 31
2.3. Equipos de esterilización
2.3.1. Autoclave
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 39.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 7
2.3.2. Equipos de esterilización por radiación
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 89-94
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 10
2.3.3. Otros medio de esterilización
12. Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 90
2. Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 7-10
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3. Técnicas de asepsia
3.1. Tècnicas de asépcia: material vegetal
3.1.1. Material vegetal (interno y externo)
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 315
Hartmann, H.T., Kester, D.E., Davies, F.T.(1990). Plant Propagation, Principles and Practices, Fifth Edition. Regents prentice Hall, Englewood Cliffs, New jersey 07632. Printed in Unites States of America, 647.
Debergh, P. C and Zimmerman R. H. (1991). Micropropagation, Technology and Application. Ed, by Debergh, P. C and Zimmerman R. H. 483 p. Publisher by Kluwer Academic Publisher . P.O. Box 17, 3300 AA. Dordrecht, The Netherlands, p. 31
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia, p. 21-23.
3.1.2. Medios Nutrientes
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 36
3.1.3. Aire
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. p. 7.
Debergh, P. C and Zimmerman R. H. (1991). Micropropagation, Technology and Application. Ed, by Debergh, P. C and Zimmerman R. H. 483 p. Publisher by Kluwer Academic Publisher . P.O. Box 17, 3300 AA. Dordrecht, The Netherlands, p. 17-23
3.1.4. Laboratorio
3.2. Medios nutrientes: agua macronutrientes, micronutrientes y azúcares
3.2.1. Componentes del medio
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 12=53.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 45-82.
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia., p. 14-19.
3.3. Aire
3.3.1. Atmósfera de los contenedores
Debergh, P. C and Zimmerman R. H. (1991). Micropropagation, Technology and Application. Ed, by Debergh, P. C and Zimmerman R. H. 483 p. Publisher by Kluwer Academic Publisher . P.O. Box 17, 3300 AA. Dordrecht, The Netherlands, p. 17-21
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4. Medio de cultivo
4.1. Nutrientes: agua, macronutrientes, micronutrientes y azucares
4.1.1. Nutrientes:agua, macronutrientes, micronutrientes y azucares
Debergh, P. C and Zimmerman R. H. (1991). Micropropagation, Technology and Application. Ed, by Debergh, P. C and Zimmerman R. H. 483 p. Publisher by Kluwer Academic Publisher . P.O. Box 17, 3300 AA. Dordrecht, The Netherlands. p. 54-57
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P.47
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports,36-39
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia, p. 15-18
4.2. Substancias orgánicas
4.2.1. Sustancias orgánicas
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 31-33
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 45-54
4.3. Concentraciones de los componentes de los medios de cultivo
4.3.1. Componentes de los medios de cultivo
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 12-51
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 45-54
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. p. 21-27
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia, p. 15-19.
4.4. Estados físicos de los medios de cultivo
4.4.1. Componentes del medio de cultivo
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 54-57
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 12-20
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia, p. 15-19.
4.5. Laboratorio
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5. Factores nutricionales y ambientales
5.1. Diferentes materiales vegetales experimentales
5.1.1. Genotipo
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports,p.4,479.
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. p. 55,64,87,89,143-144,153,156,161-162,193
Pierik, R. L .M., (1990). Cultivo in vitro de plantas superiores. Ed. Multi-prensa, p. 107-113
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 57.
5.1.1. Factores nutricionales y ambientales
5.1.2. Edad de la planta
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 81-87
5.1.3. Tipo de tejido u órgano
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 96-103
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 107, 115..
5.1.4. Células de diferente edad
Pierik, R. L .M., (1990). Cultivo in vitro de plantas superiores. Ed. Multi-prensa, p. 126-145
5.1.5. Estado fisiológico
O’ Neil Sharman D. and Robert Jeremy A. (2002). Plant Reproducction, printed, Sheffield Academic Press Ltd. Mansion House. 19 Kingfield Road, Sheffield S 11 9 AS, UK. 300 p.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 55-5.7
5.1.6. Condiciones de crecimiento
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, p. 340
Pierik, R. L .M., (1990). Cultivo in vitro de plantas superiores. Ed. Multi-prensa.p. 434
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 55-69
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y producción vegetal, p. 23-39
Serrano, M., y Piñol, M. T. 1991. Biotecnología vegetal. Ed. Síntesis, p. 37-47
5.1.7. Posición dentro de la planta del material a cultivar
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 80-95.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 107,115.
5.1.8. Fuente de crecimiento y desarrollo in vitro
R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 159.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 115.
5.2. Factores de crecimiento fisiológico
5.2.1. Luz
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 154-155.
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 121
5.2.2. Temperatura
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 142.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, 69.
5.2.3. pH
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 92.
Green, C:E. Somers, D:A:, Hackett, W:P:, Biesboer, D-D. eds. (1978). Plant Tissue and Cell Culture, Alan, R., Liss, INC. International Congress on plant tissue and Cell culture (6th: 1986: University of Minnesota) Plant Cell and Cell Culture (Plant Biology: v 3.Printed In the united states of América, Library of Concress Cataloging in publication, Data., p. 50.
5.2.4. O2 y CO2
2. Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 9, 13.
5.2.5. Humedad
5.3. Diferentes materiales vegetales experimentales
5.3.1. Luz
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 115, 149..
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 69.
5.3.2. Temperatura
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p, 68.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 112-125..
5.4. Método de inoculación
5.4.1. Efecto de las condiciones de cultivo del explante
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 55..
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 55-62.
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 8,35.
5.4.2. Preparación de las muestras antes de inocular
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 57.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 344.
5.5. Laboratorio
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6. Factores de crecimiento fisiológico
6.1. Factores de crecimiento fisiológico
6.1.1. Luz
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports, p 467-470.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands. p.139-140.-69.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherland, p. 68-69.
6.1.2. Temperatura
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands. p. 68.
6.1.3. pH
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 50.
6.1.4. O2 y CO2
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p.16,52,54.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 58, 206.
6.1.5. Humedad
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 52.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 124.
6.2. Metodos de inoculación
6.2.1. Efectos de las condiciones de cultivo del explante
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p 55-62.
6.2.2. Preparacion de las muestras antes de inocular ( forma de colecta, pretratamiento,con fitohormonas u otras condiciones)
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 107.
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 96.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p.55
6.3. Reguladores de crecimiento: Auxinas
6.3.1. Fitoreguladores
6.3.2. Auxinas
Liz, A.R. (1987). Plant Growth regulators and the control of growth and differentiation in plant tissue cultures. In Green, C:E. Somers, D:A:, Hackett, W:P:, Biesboer, D-D. eds. (1978). Plant Tissue and Cell Culture, Alan, R., Liss, INC. International Congress on plant tissue and Cell culture (6th: 1986: University of Minnesota) Plant Cell and Cell Culture (Plant Biology: v 3.Printed In the united states of América,Library of Concress Cataloging in publication, Data.p. 135-149..
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 34, 35.
Cook, T.J. and Cohen, .D. (1993). The role of auxin in Plant Embryogenesis. The plant cell, 1494-1945.
6.4. Citocininas
6.4.1. Citocininas
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 37.
6.5. Giberelinas
6.5.1. Giberelinas
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 40.
6.6. ABA
6.6.1. Acido abscisico (ABA)
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 40.
6.7. Otros reguladores de crecimiento
6.7.1. Otros promotores sintéticos y otros naturales
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 42.
6.7.2. Etileno y otros reguladores gaseosos
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 41.
6.8. Fitoreguladores Seminario
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7. Técnicas de cultivo in vitro
7.1. Cultivo de plantas intacta
7.1.1. Cultivo de plantas intactas
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 315
Green, C:E. Somers, D:A:, Hackett, W:P:, Biesboer, D-D. eds. (1978). Plant Tissue and Cell Culture, Alan, R., Liss, INC. International Congress on plant tissue and Cell culture (6th: 1986: University of Minnesota) Plant Cell and Cell Culture (Plant Biology: v 3.Printed In the united states of América,Library of Concress Cataloging in publication, Data. p. 3-15.
Torpe, A.T. ed. (1995). In vitro Embryogenesis in Plants, v. 20. Kluwer Academic Publisher Dordrech /Boston /London, Printed in the Netherlands, p. 165-166.
7.1.2. Técnicas de cultivo de tejidos
7.2. Cultivo de embriones
7.2.1. cultivo de embriones cigóticos
Torpe, A.T. ed. (1995). In vitro Embryogenesis in Plants, v. 20. Kluwer Academic Publisher Dordrech /Boston /London, Printed in the Netherlands,p. 117.
Gamborg, O. L., and Phillips G. C. ( 1995). I. Plant Cell, Tissue and Organ Culture, Fundamental Methods. Spring Berlang Berling Heidelberg. Printed in Germany. P. 131-141.
Bongas J. M. (1992). In vitro culture of Tree/ Bongas J. M. and P. Von Aderkas Publisher by kluwer Academic Publisher, P.O. Box 17, 3300 AA Dordrecht. The Netherlands, p. 126,
13. Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands, p. 139.
7.3. Cultivo de órganos
7.3.1. cultivo de órganos in vitro
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p. 20, 51-52.
Hertman, E.B. (2002), Regeneration and Micropropagation techniques Media and applications, 1999-2002. Recent advances in plant tissue cuture. Vol. 7 Agricell Reports,p. 256-299..
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands. p. 190-210..
7.4. Cultivo de callos
7.4.1. Cultivo de callos a partir de diferentes explantes
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands. p. 217-227..
7.5. Órganogénesis
7.5.1. organo genesis vegetal in vitro
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p, 29-31
7.6. Embriogénesis
7.6.1. Embriogenesis somática
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p. 33-36.
Torpe, A.T. ed. (1995). In vitro Embryogenesis in Plants, v. 20. Kluwer Academic Publisher Dordrech /Boston /London, Printed in the Netherlands, p. 155.
7.6.2. Embriogenesis cigótica
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p.49-53
Torpe, A.T. ed. (1995). In vitro Embryogenesis in Plants, v. 20. Kluwer Academic Publisher Dordrech /Boston /London, Printed in the Netherlands, p. 73.
O’ Neil Sharman D. and Robert Jeremy A. (2002). Plant Reproducction, printed, Sheffield Academic Press Ltd. Mansion House. 19 Kingfield Road, Sheffield S 11 9 AS, UK. p. 154..
7.7. Formas de cultivo in vitro en plantas superiores,: cultivo de células
7.7.1. Formas de cultivo in vitro en plantas superiores
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p, 26.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands. p 74-95..
7.7.1. cultivo de células
7.7.2. Organizada
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p. 26.
Green, C:E. Somers, D:A:, Hackett, W:P:, Biesboer, D-D. eds. (1978). Plant Tissue and Cell Culture, Alan, R., Liss, INC. International Congress on plant tissue and Cell culture (6th: 1986: University of Minnesota) Plant Cell and Cell Culture (Plant Biology: v 3.Printed In the united states of América,Library of Concress Cataloging in publication, Data.p. 57-81.
7.7.3. No organizada
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlandsp. 221-223..
7.7.4. No organizada/ organizada
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands. p.. 217-221
7.8. Cultivo de protoplastos
7.8.1. Protoplastos
Rossel, C.H., y Villalobos, V.M. eds. (1990). Fundamentos Teóricos-Prácticos del cultivo de Tejidos Vegetales. Estudio FAO producción y protección vegetal, Organización de las Naciones Unidas para la Agricultura y la Alimentación, Via dele Terme di Caracalla, 00100 Roma, Italia.p. 26, 61-65.
Pierik, R.L.M., (1987). In Vitro Culture of Higher Plant. by Martinus Nijhoff publishers, Dordrech. Printed in Netherlands., p. 266-276
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